Automated Air-Liquid Interface Cell Culture Analysis Using Deep Optical Flow

Autogenerated Report: RAINBOW Air-Liquid Interface Cell Culture Analysis

Website: https://github.com/AlphonsG/Rainbow-Optical-Flow-For-ALI

Source directory

/mnt/hdd2/Alphons/Rainbow-Optical-Flow-For-ALI/examples/example_image_series/tif_191018_HNA-ALI_d14.nd2_-_191018_HNA-ALI_d14.nd2_(series_03)0000_etc

Image Series Type

.tif

RAINBOW Analysis Timestamp

10 March 2022, 15:57:34

Image Series Metadata

{
    "analysis_timestamp": "10 March 2022, 15:57:34",
    "calibration_um": 0.31302569743655434,
    "dir": "examples/example_image_series",
    "type": ".tif"
}

Magnitude Statistics

	frame	min (um)	max (um)	mean (um)	std (um)	var (um)
0	0	0.001920	4.178234	2.138720	0.771738	0.595579
1	1	0.001551	3.935667	2.059078	0.799700	0.639520
2	2	0.102878	4.091494	2.039340	0.814543	0.663481
3	3	0.003530	3.479425	1.967105	0.759979	0.577568
4	4	0.004051	3.422691	1.889933	0.741848	0.550339
5	5	0.001329	3.128323	1.746436	0.634321	0.402363
6	6	0.005250	3.086464	1.701845	0.645827	0.417092
7	7	0.004330	3.372861	1.699563	0.644477	0.415350
8	8	0.002669	3.382966	1.754328	0.715596	0.512077
9	9	0.002633	3.494043	1.703855	0.734561	0.539580
10	10	0.003294	3.258862	1.729070	0.700330	0.490462
11	11	0.001932	3.233232	1.721925	0.726743	0.528155
12	12	0.000835	3.261815	1.664737	0.703804	0.495340
13	13	0.001779	3.125796	1.658646	0.718937	0.516870
14	14	0.002710	3.131470	1.644901	0.714538	0.510565
15	15	0.005129	3.323300	1.636716	0.735912	0.541567
16	16	0.001494	3.345547	1.656139	0.709268	0.503061
17	17	0.001446	3.342794	1.668112	0.729482	0.532144
18	18	0.000373	3.507166	1.661115	0.741280	0.549495

Direction Statistics

	frame	min (deg)	max (deg)	mean (deg)	std (deg)	var (deg)
0	0	0.000098	360.000000	43.281408	42.713049	31.841866
1	1	0.000432	359.999237	35.513476	50.172646	43.935075
2	2	0.000003	359.999969	43.784291	50.391493	44.319190
3	3	0.000375	359.999969	36.392191	55.718097	54.183858
4	4	0.000753	359.999908	30.522460	63.856827	71.169193
5	5	0.000533	359.999969	34.993708	81.487163	115.892616
6	6	0.000154	359.999969	42.838753	88.519526	136.758879
7	7	0.000137	359.999969	29.267093	88.033618	135.261583
8	8	0.000022	359.999969	25.796501	83.577503	121.914723
9	9	0.000117	359.999725	18.781743	96.761745	163.412303
10	10	0.000212	359.999939	27.118338	92.455574	149.191323
11	11	0.000289	359.999878	10.934704	95.589848	159.478047
12	12	0.000058	359.999359	19.917708	100.345852	175.742263
13	13	0.000005	359.999786	38.717613	95.348774	158.674665
14	14	0.000228	359.998840	26.982311	95.167648	158.072397
15	15	0.000116	359.999878	45.586217	95.688977	159.808983
16	16	0.000045	359.999084	26.400442	93.869449	153.789224
17	17	0.000104	359.999969	47.943119	86.507444	130.612376
18	18	0.000290	359.999908	42.746733	95.632867	159.621621

Magnitude Scatter Plot

<Figure size 432x288 with 0 Axes>

Direction Scatter Plot

<Figure size 432x288 with 0 Axes>

Raw Image Series (left) and RAINBOW Optical Flow Visualisation (Right)

a) The direction of motion at any position within RAINBOW generated optical flow images is measured clockwise from the initial horizontal position of a unit circle (left) and is shown using hue values (right).

Magnitude Heatmaps Across Image Series

Visualization of motion magnitiude using heatmap with hot colour mapping.

Quiver Plots Across Image Series

Visualisation of optical flow using quiver plots containing vector arrows.

Polar Plots Across Image Series

Visualisation of optical flow using polar plots.